Category «Biotechnology»

AIPMT PMT Bioreactors and Biotechnology MCQs

1. A bioreactor is (a) fermentation tank (b) culture containing radioactive isotopes (c) culture for synthesis of new chemicals (d) hybridoma. 2. Blunt ends of passenger and vehicle DNA’s are joined by (a) DNA polymerase I (b) RNA polymerase (c) DNA ligase (d) DNA polymerase III. 3. Restriction endonucleases are obtained from (a) bacteria (b) …

Recombinant DNA Technology

1. Isolation of the genetic material (DNA) • In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. • The DNA enclosed within the membranes has to be released by breaking the nuclear and cell membranes, along with other macromolecules such as RNA, proteins, polysaccharides …

Introduction of recombinant DNA into a suitable host

A. Increase competence of host for transformation • Competent host is essential for transformation with the recombinant DNA. Transformation is a process by which a cell takes up the naked DNA fragment from its environment, incorporates it into its own chromosomal DNA, and finally expresses the trait controlled by the incoming DNA. • Finally, the …

Enzymes in Recombinant DNA Technology

Different kinds of specific enzymes are used in genetic engineering (recombinant DNA technology). These include lysing enzymes, cleaving enzymes, synthesizing enzymes, joining enzymes and alkaline phosphatase. • The palindromes in DNA are base pair sequences that are the same, when read forward (left to right) or backward (right to left), from a central axis of …

AIPMT PMT – Biotechnology – Principles and Processes

Biotechnology • The term biotechnology is derived from a fusion of biology and technology. Biotechnology means any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use. • Berg is often considered “Father of genetic engineering”. He was awarded Nobel Prize in 1980. • …

4 Top Issues with Applications of Biotechnology

Following are the issues related to applications of Biotechnology: Ethical Issues: Ethics include a set of standards by which a community regulates its behaviour and decides as to which activity is legitimate and which is not. Therefore, bioethics may be viewed as a set of standards that may be used to regulate our activities in …

Transgenic Animals

Animals that have had their DNA manipulated to possess and express an extra (foreign) gene are known as transgenic animals or Genetically Engineered Animals. Various applications of transgenic animals are: • Study of normal physiology and development: Transgenic animals can be specifically designed to allow the study of how genes are regulated, and how they …

Applications of Biotechnology in Medicine

The recombinant DNA technological processes have made great impact in the area of healthcare by mass production of safe and more effective therapeutic drugs. A large number of human genes encoding pharmaceutically valuable proteins have been cloned and expressed in microorganisms largely employed by biotech pharmaceuticals. Initially, E. coli was used as the host for …

Biotechnology and its applications

Biotechnology is the use of living systems and organisms to develop or make useful products. It harnesses cellular and biomolecular processes to develop technologies and products that help improve our health and lives. Modern biotechnology provides breakthrough solutions and technologies to combat debilitating and rare diseases, reduce our environmental footprint, feed the hungry, use less …