1. A bioreactor is
(a) fermentation tank
(b) culture containing radioactive isotopes
(c) culture for synthesis of new chemicals
2. Blunt ends of passenger and vehicle DNA’s are joined by
(a) DNA polymerase I (b) RNA polymerase
(c) DNA ligase (d) DNA polymerase III.
3. Restriction endonucleases are obtained from
(a) bacteria (b) all prokaryotic cells
(c) bacteriophages (d) plasmids.
4. Enzyme Taq polymerase is obtained from
(a) Thermus aquaticus (b) Trichoderma aquatica (c) Tremetes aquaticus (d) all of these.
5. Genetic engineering is possible, because
(a) we can cut DNA at specific sites by endonucleases like DNAase I
(b) restriction endonucleases purified from bacteria can be used in vitro
(c) the phenomenon of transduction in bacteria is well understood
(d) we can see DNA by electron microscope.
6. Two microbes found to be very useful in genetic engineering are
(a) crown gall bacterium and Caenorhabditis elegans
(b) Escherichia coli and Agrobacterium tumefaciens
(c) Vibrio cholerae and a tailed bacteriophage
(d) Diplococcus sp. and Pseudomonas sp.
7. Which enzyme is used as ‘molecular scissor’ in genetic engineering?
(a) restriction endonuclease
(b) DNA polymerase
(c) DNA ligase
(d) DNA gyrase
8. Polymerase chain reaction (PCR) is used for
(a) DNA identification
(b) DNA repair
(c) DNA amplification
(d) DNA cleavage.
9. Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only little DNA could be extracted from it. If the genes of the ancient man need to be analysed, the best way of getting sufficient amount of DNA from this extract is
(a) subjecting the DNA to gel electrophoresis
(b) treating the DNA with restriction endonucleases
(c) hybridising the DNA with a DNA probe
(d) subjecting the DNA to polymerase chain reaction.
10. A technique used in making copies of a specific segment of DNA involves
(c) polymerase chain reaction
(d) ligase chain reaction.
11. Where is NDRI located?
(a) Delhi (b) Lucknow
(c) Karnal (d) Hisar
12. The process of making temporary/transitory pores in the cell membrane to introduce foreign DNA is
(c) particle gun
(d) chemical mediated genetic transformation.
13. EcoRI cleaves DNA at
(a) AAGGTT (b) GTATATC
(c) GAATTC (d) TATAGC.
14. Gel electrophoresis is used for
(a) construction of recombinant DNA by joining with cloning vectors
(b) isolation of DNA molecules
(c) cutting of DNA into fragments
(d) separation of DNA fragments according to their size.
15. Identify the plasmid
(a) EcoRI (b) pBR322
(c) AluI (d) Hindlll.
16. First hormone prepared by genetic engineering is
(a) oxytocin (b) somatotropin
(c) adrenalin (d) insulin.
17. During “gene cloning” which is called a gene taxi?
(a) vaccine (b) plasmid
(c) bacteria (d) protozoa
18.One of the key factors, which makes the plasmid the vector in genetic engineering is that
(a) it is resistant to antibiotics
(b) it is resistant to restriction enzymes
(c) its ability to carry a foreign gene
(d) its ability to cause infection in the host.
19. Which of the following is not a means of genetic transfer?
(a) conjugation (b) transduction
(c) induction (d) transformation
20. Who discovered recombinant DNA (rDNA) technology?
(a) HarGobind Khorana
(b) James D. Watson
(c) Stanley Cohen and Herbert Boyer
(d) Walter Sutton and Avery