A Hitch Hiker’s guide to the Basic Onion Experiment with Microscope

Do you want to confine your life in the miniature world of microscopy? Are you a loner who wants an excuse to skip a social function? Or does being called a nerd makes your identity? Yes, we know that anticipation. You’re struggling hard to find something unusual to search on your microscopy but you’re running short of ideas. Are we right? Yes, we get you right. Dear friend, everything comes with a price tag and so do the feeling of accomplishment. Hard work, concentration, dedication and focus can get you through the ladder of success. Mind well, oh curious spectator, there is no shortcut to success. So fasten up your seat belts and get ready to get what you deserve. To get you started with your business, we’ve calibrated some of the neat examples to polish up your trial and test traits. No matter how amateur you consider yourself to be, the experiments are bound to get neat, precise and error free results. That’s our insurance, fella aspirant! Get set go!

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Onion slide Experiment:

We’re back to basics. Yes, we’re talking about the vegetable that has graced your kitchen ever since you were born. It is none other than onion. Had it not been for onion, our food wouldn’t have been so delicious. Let’s study the stem cells of onion while you get to know what an onion look like at a microscopic level. It will hardly take five minutes of your time.

Step 1:

Sprinkle water on your microscope slide to get it moist. Take an onion from your kitchen and peel of the cell membrane of the onion skin and keep it under microscope for observation.

Step2:

If you think the step above is all what you need to do, then you may get your facts right. Make sure there are no air bubbles formed to pollute your result. If you find one, burst the bubble with a pin. 

Step 3:

The lens, closer to the objective lens, has to be collinear with the optical tube so as to provide maximum illumination and glitch free results.

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Step 4:

Now that you’re done with all prior arrangements, let us fine tune your observation. That being said, adjust your tube by looking through the eyepiece at a conical angle. This is called coarse tuning. It will bound to affect your results at large. After you’re done with the coarse tuning, your next move should to fine tune by slightly moving fine tune knob. It is meant to enhance your results by adding perfection.  

Step 5:

After you’ve successfully tuned it, an image would come to focus. Pat your back and self-congratulate yourself. You’re quarter way through. The only thing to do after you’ve retrieved the image is to magnify it. And by the way, don’t look surprised if you catch something like lizard’s skin under scanner.

Step 6:

Try the same experiment using a dye solution as a supplement in order to figure out the nucleus of each cell.

We wish that this tutorial will be deemed fruitful to you. For a start, this is the best exercise that you’ll practice as an amateur.

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