Characteristics of a cloning vector in Recombinant DNA Technology

cloning-vector

Origin of replication (Ori)

• This is a specific sequence from where replication starts, when any piece of foreign DNA is linked to this sequence.
• The replication occurs inside the host cells. This sequence is also responsible for controlling the copy number of linked DNA.
• A prokayotic DNA has single origin of replication, while eukaryotic DNA may have more than one origin of replication.
Selectable markers

• The vector also requires a selectable marker (antibiotic resistance gene) to identify and eliminate non transformants and selectively permit the growth of the transformants.

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