Characteristics of a cloning vector in Recombinant DNA Technology


Origin of replication (Ori)

• This is a specific sequence from where replication starts, when any piece of foreign DNA is linked to this sequence.
• The replication occurs inside the host cells. This sequence is also responsible for controlling the copy number of linked DNA.
• A prokayotic DNA has single origin of replication, while eukaryotic DNA may have more than one origin of replication.
Selectable markers

• The vector also requires a selectable marker (antibiotic resistance gene) to identify and eliminate non transformants and selectively permit the growth of the transformants.

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Comments ( 2 )
  1. Introduction of recombinant DNA into a suitable host | Hourly Book
    February 22, 2015 at 7:48 pm

    […] been used to : introduce the recombinant DNA into recipient cells of ; animals, without involving a cloning vector. Microinjection • In this method, foreign DNA is directly injected into the nucleus of animal or […]

  2. rafa
    February 24, 2015 at 5:35 pm

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