AIPMT Biotechnology Multiple Choice Questions

Part I
1. The crops having cry genes need
(a) no insecticide
(b) small amount of insecticide
(c) large amount of insecticide
(d) none of the above
2. Golden rice is
(a) a type of rice grown along the Yellow river in China
(b) a transgenic rice having gene for p-carotene (pro-vitamin-A)
(c) normal variety of rice with golden coloured grains
(d) wild and long sized rice having golden tint
3. Bt tobacco was first cultured to kill
(a) hornworm (b) bollworm
(c) stem borer (d) tobacco budworm
4. Main objective of the production/use of herbicide resistant GM crops is to
(a) eliminate weeds from the fields without any use of manual labour
(b) eliminate weeds from the fields without any use of herbicides
(c) encourage eco-friendly herbicides
(d) reduce herbicide accumulation in food articles for health safety
5. Read the following statements and select the correct ones.
I. In 1997,anAmericancompanygotpatentrightsfor Basmati rice through the US Patent and Trademark office and was allowed to sell a ‘new variety’ in US and abroad.
II. This new variety of Basmati was derived from Chinese farmer’s varieties.
III. Indian Basmati was crossed with semidwarf
varieties and claimed as an invention or a novelty.

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(a) I and II (b) I and III
(c) II and III (d) I, II and III
6. Some organizations and multinational companies patent biological resources of other nations without proper authorization from the countries concerned, this is called
(a) bioweapon (b) biopiracy
(c) biothics (d) biopatent
7. Which gene was introduced in the first transgenic cow?
(a) Human oc-lactalbumin
(b) oc-1-antitrysin
(c) p-1-antitrypsin
(d) cry-IAc
8. What might be the advantage of beginning gene therapy prior to birth?
(a) This would give the body plenty of time
(b) The body would not reject it as it has not yet recognised ‘self’.
(c) The cells being extremely young are more receptive of gene therapy
(d) None of these
9. Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
(a) Splitting A and B-Peptide chain
(b) Addition of C-peptide to proinsulin
(c) Getting insulin assembled into mature form
(d) Removal of C-peptide from active insulin
10. Bt toxin kill insects by
(a) inhibiting protein synthesis
(b) generating excessive heat
(c) creating pores in the midgut epithelial cells, leading to cell swelling and lysis
(d) obstructing a biosynthetic pathway
11. What is the demerit of using bovine insulin (from cow) and porcine insulin (from pig) in diabetic patients?
(a) it leads to hypercalcemina
(b) it is expensive
(c) it may cause allergic reactions
(d) it may lead to mutation in human genome
12.ELISA is based on
(a) antigen-antibody interaction
(b) antigen-protein interaction
(c) lectin-antibody interaction
(d) All of the above

13. PCRis used to
(a) detect HIV in suspected AIDS patients
(b) detect mutations in the genes in suspected cancer patients
(c) diagnose many genetic disorders
(d) all of the above
14. A USA patent was taken for
(a) Basmati rice (b) Lerma Roja
(c) CO-668 (d) Sharbati Sonara
15. Bt corn has been made resistant from corn borer disease by the indroduction of the gene
(a) cry I Ac (b) cry II Ab
(c) cry I Ab (d) cry II Ac
Part II
1. RNA interference involves
(a) synthesis of cDNA and RNA using reverse transcriptase
(b) silencing of specific mRNA due to complementary RNA
(c) interference of RNA in synthesis of DNA
(d) synthesis of mRNA from DNA.
(NEET – Karnataka 2013)
2. Which one of the following vectors is used to replace the defective gene in gene therapy?
(a) Adenovirus (b) Cosmid
(c) Ri plasmid (d) Ti plasmid
(NEET – Karnataka 2013)
3. Genetically modified (GM) crops can be produced by
(a) recombinant DNA technology
(b) somatic hybridisation
(c) crossbreeding
(d) micropropagation. (AIIMS 2013)
4. Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin ‘A’ deficiency?
(a) ‘Flavr Savr’tomato (b) Canolla (c) Golden rice (d) 8f-Brinjal.
(AIPMT Prelims 2012)
5. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produces (in the host cells)
(a) both sense and anti-sense RNA
(b) a particular hormone
(c) an antifeedant
(d) a toxic protein. (AIPMT Mains 2012)
6. Read the two statements A and B and identify the correct circle from those given below.
Statement A : Agrobacterium tumefaciens is the causative agent of crown gall disease of dicots.
Statement B : Agrobacterium tumefaciens causes infection by entering the plant through wounds and injuries.
(a) Statement A is correct and B is incorrect.
(b) Statement B is correct and A is incorrect.
(c) Both statements A and B are correct.
(d) Both statements A and B are incorrect.
(Karnataka 2012)
7. Which of the following is known as ‘Flavr Savr’?
(a) Specific variety of pesticide
(b) Breed of chicken
(c) Transgenic tomato
(d) Toxic insecticidal protein. (Odisha 2012)
8. Human proteins can be produced in the milk or semen of farm animals. True or false?
(a) True.
(b) False, proteins cannot be produced in milk.
(c) False, proteins cannot be produced in semen.
(d) False, animals are not used for protein production.
(J & K 2012)
9. Maximum number of existing transgenic animals is of
(a) fish (b) mice
(c) cow (d) pig
(AIPMT Prelims 2011)
10. Bacillus thuringiensis forms protein crystals which contain insectidical protein. This protein
(a) binds with epithelial cells of midgut of the insect pest ultimately killing it.
(b) is coded by several genes including the gene cry.
(c) is activated by acid pH of the foregut of the insect pest.
(d) does not kill the carrier bacterium which is itself resistant to this toxin. (AIPMT Mains 2011)
11. During the processing of the prohormone “proinsulin” into the mature “insulin”
(a) C-peptide is added to proinsulin
(b) C-peptide is removed from proinsulin
(c) B-peptide is added to proinsulin
(d) B-peptide is removed from proinsulin.
(AMU 2011)
12. In hydridoma technology
(a) B-cells are fused with myeloma cells
(b) T-cells are fused with myeloma cells
(c) B-cells are fused with T-cells
(d) none of the above. (AMU 2011)
13. Genetic engineering has been successfully used for producing
(a) transgenic mice for testing safety of polio vaccine before use in humans
(b) transgenic models for studying new treatments for certain cardiac diseases
(c) transgenic cow-Rosie which produces high fat milk for making ghee
(d) animals like bulls for farm work as they have super power. (AIPMT Prelims 2010)
14. Some of the characteristics of Bt cotton are
(a) long fibre and resistance to aphids
(b) medium yield, long fibre and resistance to beetle pests
(c) high yield and production of toxic protein crystals which kill dipteran pests
(d) high yield and resistance to bollworms.
(AIPMT Prelims 2010)
15. An improved variety of transgenic basmati rice
(a) does not require chemical fertilizers and growth hormones
(b) gives high yield and is rich in vitamin A
(c) is completly resistant to all insect pests and diseases of paddy
(d) gives high yield but has no characteristic aroma.
(AIPMT Prelims 2010)
16. ‘Gray biotechnology’ is referred to
(a) medical process (b) industrial process (c) agricultural process (d) aquatic process.
(AMU 2010)
17. Which of the following is used as a bioweapon?
(a) Clostridium botulinum
(b) Escherichia coli
(c) Bacillus anthracis
(d) both (a) and (c) (BHU 2008)
18. Transgenic plants are the ones
(a) generated by introducing foreign DNA into a cell and regenerating a plant from that cell
(b) produced after protoplast fusion in artificial medium
(c) grown in artificial medium after hybridization in the field
(d) produced by a somatic embryo in artificial medium. (AIIMS 2009)
19. The polymerase chain reaction is a technique that
(a) is used for in vivo replication of DNA
(b) is used for in vivo synthesis of mRNA
(c) is used for in vitro synthesis of mRNA
(d) used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.
(Odisha 2008)
20. Which one of the following is a correct statement?
(a) “Bt” in “B/-cotton” indicates that it is a genetically modified organism produced through biotechnology.
(b) somatic hybridization involves fusion of two complete plant cells carrying desired genes.
(c) the anticoagulant hirudin is being produced from transgenic Brassica napus seeds.
(d) “flavr savr” variety of tomato has enhanced the production of ethylene which improves its taste.
(AIIMS 2005)



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